Cairan ionik diekstraksi dengan microwave-assistedextraction dengan kondisi: konsentrasi Cairan ionik 1 mol/L, Rasio padatcair 1:20 (g/mL), waktu ekstraksi 10 menit dan power 30% (270 Watt). Metode ekstraksi konvensional yang dilakukan, yaitu refluks dan microwave-assisted extraction. Jenis pelarut organik yang digunakan yaitu etanol 65% dan cairan ionik. Serbuk daun komfrey diekstraksi dengan berbagai kondisi. (BMIM) Br extraction with MAE can attract the highest retrorsine N-oxide in comfrey leaves.Ībstrak: Ekstraksi senyawa bioaktif daun komfrey ( Symphytum officinale L.) dilakukan dengan membandingkan pelarut organik dan cairan ionik, antara metode konvensional dengan metode microwave assisted extraction. Extraction of comfrey leaves using the MAE method of 65% ethanol produced the highest total phenolic and allantoin content compared to other methods. The highest levels of retrorsine N-oxide were obtained by extraction of MAE with (BMIM)Br with a concentration of 0.049 ± 0.007. The highest levels of allantoin were also obtained from MAE extraction with 65% ethanol solvent at 2.922 ± 0.123. MAE extraction in a solvent containing 65% ethanol resulted in the highest total phenolic content of 1.038☐.012. Allantoin and retrorsine N-oxide levels were analyzed by thin-layer chromatography – densitometry. The total phenolic content of the extract was analyzed with a microplate reader.
The ionic liquid was extracted by microwave-assisted extraction with the following conditions: ionic liquid concentration of 1 mol/L, the solid-liquid ratio of 1:20 (g/mL), extraction time of 10 minutes, and power of 30% (270 Watt). The extraction methods used are reflux and microwave-assisted extraction. The types of organic solvents used are 65% ethanol and ionic liquids. The comfrey leaf powder was extracted under various conditions.
Extraction of bioactive compounds from comfrey leaves ( Symphytum officinale L.) was carried out by comparing organic solvents and ionic liquids between conventional and microwave-assisted extraction (IL-MAE) methods.